Karol S Bruzik
Professor
Associate Head for Curricular Affairs
Pharmaceutical Sciences
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About
Research interests: Bioorganic chemistry. Investigation of mechanisms of inositol-related enzymes and their function in cell signaling events. Synthesis of analogs of biophosphates as inhibitors and probes of enzyme mechanisms. Real-time, live-cell assay of enzymatic activities in response to receptor stimulation. Isolation, structure determination and synthesis of novel phosphoinositide second messengers.
About
Teaching and Supervision
Doctoral Restch in Med Chem (MDCH 599), 1/9/2023 – 4/28/2023
Biomedicinal Chemistry (PHAR 423), 1/9/2023 – 4/28/2023
Biomedicinal Chemistry (PHAR 423), 1/9/2023 – 4/28/2023
Biomedicinal Chemistry (PHAR 423), 1/9/2023 – 4/28/2023
Biomedicinal Chemistry (PHAR 423), 1/9/2023 – 4/28/2023
Undergrad Res Pharm Sci (PSCI 380), 8/22/2022 – 12/2/2022
Special Proj Pharm Sci (PSCI 390), 8/22/2022 – 12/2/2022
Introduction to Pharmaceutical Sciences (PHAR 200), 8/22/2022 – 12/2/2022
Doctoral Restch in Med Chem (MDCH 599), 8/22/2022 – 12/2/2022
Selected Grants
Molecular Pharmacology of the Synaptic and Extrasynaptic GABA(A) Receptors, Massachusetts General Hospital., 4/1/2020 - 2/29/2024, Obligated Amount: $778426; Anticipated Amount: $778426
DACCPM Miller Research Sundry Activities, Partners Healthcare., 3/16/2020 - 3/15/2021, Obligated Amount: $107181; Anticipated Amount: $107181
General Anesthetic Sites on Ligand-Gated Ion Channels, Massachusetts General Hospital., 9/1/2014 - 8/31/2020, Obligated Amount: $1064640; Anticipated Amount: $1064640
General Anesthetic Sites in GABA-A Receptor Subunit Interfacial Pockets, Massachusetts General Hospital., 8/1/2014 - 7/31/2017, Obligated Amount: $74648; Anticipated Amount: $74648
Nanoparticle-Based Photo-Activator of Voltage-Gated Sodium Channels, National Institutes of Health (National Eye Institute)., 5/1/2013 - 4/30/2016, Obligated Amount: $447531; Anticipated Amount: $451500
General Anesthetic Sites On Ligand-Gates Ion Channels, Massachusetts General Hospital., 9/30/2009 - 8/31/2014, Obligated Amount: $728485; Anticipated Amount: $728485
Verification Of The Results Described In US Paatent #5968976, Foley & Lardner LLP., 2/19/2007 - 3/30/2007, Obligated Amount: $13670; Anticipated Amount: $13670
Functional Composite Nanofibers By Coelectrospinning: Funtional Nanoobjects For Life Sciences, VolkswagenStiftung Fdn., 1/1/2007 - 12/31/2007, Obligated Amount: $51045; Anticipated Amount: $51045
Subcontract Grant To Cellular And Molecular Targets Of General Anestheics, Cornell University., 8/1/2006 - 7/31/2007, Obligated Amount: $340330; Anticipated Amount: $340330
Selected Publications
Koinas, Dimosthenis A, Wu, Bo, Zhou, Xiaojuan, Pajak, Anthony Y, Miller, Keith W, Bruzik, Karol S. (2022). Design and Development of Site Specific Null Allosteric Ligands for γ‐Aminobutyric Acid Type A Receptor as Reversal Agents for General Anesthesia. The FASEB Journal, 36, (S1). doi:10.1096/fasebj.2022.36.s1.r4350.
Jayakar, Selwyn S, Chiara, David C, Zhou, Xiaojuan, Wu, Bo, Bruzik, Karol S, Miller, Keith W, Cohen, Jonathan B. (2020). Photoaffinity labeling identifies an intersubunit steroid-binding site in heteromeric GABA type A (GABAA) receptors. Journal of Biological Chemistry, 295, (33), 11495-11512. doi:10.1074/jbc.ra120.013452.
Shalabi, Abdelrahman R, Yu, Zhiyi, Zhou, Xiaojuan, Jounaidi, Youssef, Chen, Hanwen, Dai, Jiajia, Kent, Daniel E, Feng, Hua-Jun, Forman, Stuart A, Cohen, Jonathan B, Bruzik, Karol S, Miller, Keith W. (2020). A potent photoreactive general anesthetic with novel binding site selectivity for GABAA receptors. European Journal of Medicinal Chemistry, 194, 112261. doi:10.1016/j.ejmech.2020.112261.
Kent, Daniel E, Savechenkov, Pavel Y, Bruzik, Karol S, Miller, Keith W. (2019). Binding site location on GABAA receptors determines whether mixtures of intravenous general anaesthetics interact synergistically or additively in vivo. British Journal of Pharmacology, 176, (24), 4760-4772. doi:10.1111/bph.14843.
Jayakar, Selwyn S, Zhou, Xiaojuan, Chiara, David C, Jarava-Barrera, Carlos, Savechenkov, Pavel Y, Bruzik, Karol S, Tortosa, Mariola, Miller, Keith W, Cohen, Jonathan B. (2019). Identifying drugs that bind selectively to intersubunit general anesthetic sites in the α1β3γ2 GABAAR transmembrane domain. Molecular Pharmacology, 95, (6), mol.118.114975. doi:10.1124/mol.118.114975.
Yu, Zhiyi, Chiara, David C, Savechenkov, Pavel Y, Bruzik, Karol S, Cohen, Jonathan B. (2019). A photoreactive analog of allopregnanolone enables identification of steroid-binding sites in a nicotinic acetylcholine receptor. Journal of Biological Chemistry, 294, (19), 7892-7903. doi:10.1074/jbc.ra118.007172.
Jayakar, Selwyn S, Zhou, Xiaojuan, Wu, Bo, Miller, Keith W, Bruzik, Karol S, Cohen, Jonathan B. (2019). Pharmacological Selectivity of a Steroid Binding Site at the Beta+-Alpha− Interface of Alpha1 Beta3 Gaba-A Receptors. Biophysical Journal, 116, (3), 392a. doi:10.1016/j.bpj.2018.11.2122.
Chiara, David C, Wu, Bo, Zhou, Xiaojuan, Jayakar, Selwyn S, Titterton, Katherine, Forman, Stuart A, Miller, Keith W, Bruzik, Karol S, Cohen, Jonathan B. (2019). Identification of a High-Affinity Neurosteroid Binding Site in Human Alpha1Beta3 GabaAR using a Photoreactive Thdoc Analog. Biophysical Journal, 116, (3), 392a. doi:10.1016/j.bpj.2018.11.2123.
Yu, Zhiyi, Savechenkov, Pavel Y, Bruzik, Karol S, Cohen, Jonathan B. (2019). Identifying Steroid Binding Sites in a Nicotinic Acetylcholine Receptor (nAChR) with a Photoreactive Analog of Alphaxalone. Biophysical Journal, 116, (3), 395a. doi:10.1016/j.bpj.2018.11.2135.
Education
Degrees:
D.Sc., Institute of Organic Chemistry, Poland, 1988
M.Sc., Technical University of Lodz, Poland, 1972
Ph.D., Polish Academy of Sciences, Poland, 1980